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Bitterness : Perception, Chemistry and Food Processing

Bitterness : Perception, Chemistry and Food Processing

autor:
Michel Aliani; Michael N. A. Eskin
 
editor:
Wiley-Blackwell
 
edição:
1
 
ano de publicação:
2017
 
idioma:
Inglês
 
ISBN:
9781118590232
 
nº de páginas:
264
 
formato:
ileio (leitura online e APP)
 

The increasing demand for healthy foods has resulted in the food industry developing functional foods with health-promoting and/or disease preventing properties. However, many of these products bring new challenges. While drugs are taken for their efficacy, functional foods need to have tastes that are acceptable to consumers. Bitterness associated with the functional foods is one of the major challenges encountered by food industry today and will remain so in years to come. This important book offers a thorough understanding of bitterness, the food ingredients that cause it and its accurate measurement.

The authors provide a thorough review of bitterness that includes an understanding of the genetics of bitterness perception and the molecular basis for individual differences in bitterness perception. This is followed by a detailed review of the chemical structure of bitter compounds in foods where bitterness may be considered to be a positive or negative attribute. To better understand bitterness in foods, separation and analytical techniques used to identify and characterize bitter compounds are also covered. 

Food processing can itself generate compounds that are bitter, such as the Maillard reaction and lipid oxidation related products. Since bitterness is considered a negative attribute in many foods, the methods being used to remove and/mask it are also thoroughly discussed.

List of Contributors xiii

Preface xv

SECTION I THE BIOLOGY OF BITTERNESS PERCEPTION 1

1 Biochemistry of Human Bitter Taste Receptors 3
Jasbir Upadhyaya, Nisha Singh, Raj Bhullar, and Prashen Chelikani

1.1 Introduction 3

1.2 Bitter taste receptors: T2Rs 3

1.3 T2R signal transduction 5

1.4 Bitter taste perception and T2R polymorphisms 6

1.5 Ligand binding and activation mechanisms of T2Rs 8

1.6 Nutrigenomics of taste 10

1.7 Bitter taste blockers 12

1.8 Expression of T2Rs in extraoral tissues 12

1.9 Conclusion 13

Acknowledgement 14

References 14

2 Physiological Aspects of Bitterness 21
Maik Behrens and Wolfgang Meyerhof

2.1 Introduction 21

2.2 Anatomy 21

2.3 Taste signal transduction 23

2.4 Gustatory bitter taste receptor gene expression 24

2.5 Extragustatory bitter taste receptors 28

2.6 Outlook 31

Acknowledgements 31

References 31

3 Bitterness Perception in Humans: An Evolutionary Perspective 37
Hui Yang and Peng Shi

3.1 Bitter taste receptors - A group of G protein-coupled receptor (GPCR) members 38

3.2 Tas2R gene family - A highly diverse family in vertebrates 38

3.2.1 Bitter taste receptors - a group of G protein-coupled receptor (GPCR) members 38

3.3 The evolution of Tas2R gene family in vertebrates 40

3.4 Diverse selective forces drove the evolution of Tas2R genes in primates 41

3.5 Genetical basis of tasteblindness – human PTC perception as an example 43

3.6 PTC tasteblindness in humans and chimpanzees - shared phenotype resulted from unshared genotypes 44

3.7 Closing remarks 45

Acknowledgement 46

References 46

SECTION II THE CHEMISTRY OF BITTERNESS 49

4 Fruits and Vegetables 51
Ernst Hoehn and Daniel Baumgartner

4.1 Introduction 51

4.2 Fruits 56

4.2.1 Flavonoids, flavonols and limonoid aglycones in grapefruit, orange and lemons 56

4.2.2 Cyanogenic glycoside in apricot, almonds and other species 58

4.3 Vegetables 60

4.3.1 Brussels sprouts, cabbage, cauliflower, turnips/Swedes and collards and kale: glucosinolates/isothiocyanates and phenolics 60

4.3.2 Carrots: 6-Methoxymellein, polyacetylenes and phenolic acids 63

4.3.3 Potatoes, tomatoes and other solanum species: glycoalkaloids 66

4.4 Future progress 68

References 68

5 Bitterness in Beverages 81
Ayyappan A. Aachary and N. A. Michael Eskin

5.1 Introduction 81

5.2 Bitterness in tea 81

5.3 Bitterness in coffee 83

5.4 Bitterness in cocoa/hot chocolate 86

5.5 Bitterness in beer 87

5.6 Bitterness in wine 90

5.7 Bitterness in cider 92

References 94

6 Structural Characteristics of Food Protein-Derived Bitter Peptides 103
Rotimi E. Aluko

6.1 Introduction 103

6.2 Bitter peptides preparation and taste evaluation 107

6.3 Role of amino acid composition and position arrangement in determining peptide bitterness intensity 108

6.3.1 Relationship between peptide hydrophobicity and bitterness intensity 108

6.3.2 Influence of peptide chain length and N- or C-terminal amino acid residue 109

6.3.3 Amino acid type and position on peptide chain 111

6.3.4 Influence of amino acid isomeric configuration 117

6.4 Peptide Debittering Methods 117

6.4.1 Peptide complexation 117

6.4.2 Hydrophobic column adsorption 120

6.4.3 Enzyme treatment 121

6.5 Conclusions 123

Acknowledgement 123

References 123

SECTION III ANALYTICAL TECHNIQUES FOR SEPARATING AND CHARACTERIZING BITTER COMPOUNDS 129

7 Sensory Evaluation Techniques for Detecting and Quantifying Bitterness in Food and Beverages 131
Donna Ryland, Erin Goldberg, and Michel Aliani

7.1 Screening methods 131

7.2 Test methods 132

7.3 Techniques to maximize bitterness perception 137

7.4 Use of standards 138

7.5 Conclusion 139

References 153

8 Analysis of Bitterness Compounds by Mass Spectrometry 159
Geraldine Dowling

8.1 Introduction 159

8.2 Overview of LC-MS 163

8.2.1 Electrospray ionisation 163

8.2.2 Solvents 164

8.2.3 Additives 164

8.2.4 pH 165

8.2.5 Adduct formation 166

8.2.6 Ion-Pairing and ion exchange 166

8.3 Data acquisition in LC-MS 167

8.3.1 Targeted compound screening 167

8.3.2 Non-targeted compound (retrospective) screening 167

8.3.3 Ion annotation 169

8.3.4 Mass-based identification 169

8.3.5 Spectral interpretation 170

8.3.6 Spectral matching 170

8.3.7 Compound identification 171

8.4 LC-MS application of bitterness compounds 172

8.4.1 Bitter compound quantitation by triple quadrupole and selected ion monitoring 172

8.4.2 Quantitation of bitter compounds by LC-IT-MS and LC-HRMS 175

8.5 Challenges and future perspectives 178

8.6 Optimisation of mass spectra parameters 179

8.7 Recording of MSn profile 179

8.8 Challenges in the collection of HRMS data 183

8.9 Conclusions 185

References 186

9 Evaluation of Bitterness by the Electronic Tongue: Correlation between Sensory Tests and Instrumental Methods 193
Michel Aliani, Ala’a Eideh, Fatemeh Ramezani Kapourchali, Rehab Alharbi, and Ronak Fahmi

9.1 Introduction 193

9.2 The electronic tongue 193

9.2.1 Sensor arrays 194

9.2.2 Data processing 195

9.3 The electronic tongue and food production 195

9.4 Electronic tongue and bitterness 197

9.5 Evaluating bitterness in food products using electronic tongues 198

9.6 Conclusion 202

References 202

SECTION IV METHODS FOR REMOVING BITTERNESS IN FUNCTIONAL FOODS AND NUTRACEUTICALS 207

10 Methods for Removing Bitterness in Functional Foods and Nutraceuticals 209
Erin Goldberg, Jennifer Grant, Michel Aliani, and Michael Eskin

10.1 Introduction 209

10.2 Reducing and removing bitter components 210

10.2.1 Physical methods 210

10.2.1.1 Temperature treatment 210

10.2.1.2 Ion exchange and adsorbent resins 222

10.2.1.3 Extraction with sub-critical water 222

10.2.2 Chemical methods 222

10.2.2.1 Fermentation 222

10.2.2.2 Aging and polymerization of phenols 223

10.2.2.3 Alkalization 223

10.2.3 Masking techniques 224

10.2.3.1 Flavorings 224

10.2.3.2 Amino acids 226

10.2.3.3 Aroma additions 226

10.2.3.4 Gluconate and acetate 228

10.2.3.5 Lipids: phospholipids and fatty acids 228

10.2.3.6 Zinc, lactate, and acetate 228

10.2.4 Bitter blockers 229

10.3 Conclusion 231

References 232

Index 237

Informação indisponível
Pessoal
Tipo de licença Permissão de impressão
Acesso Perpétuo não permitido


Leitura online: um utilizador por sessão (sem simultaneidade)
Leitura offline (com a APP): máximo de 2 dispositivos em simultâneo

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